Thermodynamic parameter values of unfolding of wild-type and chimeric BlaP.
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The values were deduced from the analysis of unfolding transitions monitored by intrinsic fluorescence and far-UV CD measurements. The urea unfolding experiments were carried out at 25°C. ΔG°NU(H2O), mNU, Cm and Tmapp values result from the mean of the values obtained by far-UV CD and intrinsic fluorescence; note that for thermal unfolding, three measurements were carried out both by fluorescence and CD. Thermal unfolding was not fully reversible and only the apparent mid-unfolding temperature (Tmapp) was determined.
创建时间：
2015-12-02
