Experimental and coastscape data on salinity responses and potential hyposaline exposure in Anomalocardia flexuosa

This dataset contains experimental and spatial data used to assess behavioral and physiological responses of the bivalve Anomalocardia flexuosa to salinity variation and to characterize potential hyposaline exposure across its known distribution along the Brazilian coast. Missing values in the valve-opening dataset indicate individuals whose valve position could not be observed because they had burrowed into the sediment. Spatial data were obtained from Bio-ORACLE, WorldClim 2.1, and HydroRIVERS and were used to characterize background salinity, precipitation patterns, river discharge, drainage characteristics, and proximity to river mouths in environmental units representing known occurrence areas of the species. burrow.csv - Individual burrowing observations recorded after 4, 24, and 28 h of exposure to nine experimental salinities. Responses are coded as “sim” (burrowed) or “não” (not burrowed). valve_4hrs.csv - Individual valve-opening observations recorded after 4 h of exposure to nine experimental salinities. Binary values indicate whether the valves were observed open or closed. valve_24hrs.csv - Individual valve-opening observations recorded after 24 h of exposure to nine experimental salinities. Missing values indicate individuals that had burrowed and whose valve position could not be observed. valve_28hrs.csv - Individual valve-opening observations recorded after 28 h of exposure to nine experimental salinities. The observation followed the repositioning of specimens with the umbo facing upward. osm.csv - Individual hemolymph osmolality measurements obtained after 28 h of exposure to nine experimental salinities. Osmolality is expressed in mOsm kg⁻¹. th.csv - Individual tissue hydration measurements obtained after 28 h of exposure to nine experimental salinities. Tissue hydration is expressed as the percentage of water relative to tissue wet mass. lactato.csv - Individual lactate concentrations measured in adductor-muscle samples after 28 h of exposure to nine experimental salinities. ldh.csv - Individual lactate dehydrogenase activity measurements obtained from adductor-muscle samples after 28 h of exposure to nine experimental salinities. prot.csv - Total protein concentrations measured in adductor-muscle homogenates and used to normalize lactate dehydrogenase activity. Bio-ORACLE v3 sea-surface salinity data used to characterize background salinity conditions across the environmental units. WorldClim 2.1 bioclimatic data used to quantify annual precipitation, wettest-quarter precipitation, and precipitation seasonality. HydroRIVERS data and derived river-mouth locations used to quantify river discharge, drainage characteristics, and the distance between occurrence areas and river mouths.本数据集涵盖实验数据与空间数据两类，用于评估波纹斧蛤（Anomalocardia flexuosa）对盐度变化的行为与生理响应，并表征其在巴西沿岸已知分布范围内的潜在低盐暴露情况。壳瓣开合数据集中的缺失值代表因潜入沉积物中而无法观测到壳瓣位置的个体。空间数据取自Bio-ORACLE、WorldClim 2.1及HydroRIVERS，用于刻画该物种已知分布区对应环境单元中的背景盐度、降水模式、河流径流量、流域特征以及与河口的邻近程度。 burrow.csv：记录了暴露于9种实验盐度下4、24及28小时后的个体潜穴观测记录，响应结果编码为"sim"（已潜穴）或"não"（未潜穴）。 valve_4hrs.csv：记录了暴露于9种实验盐度下4小时后的个体壳瓣开合观测记录，二进制值用于指示壳瓣是否被观测为张开或闭合状态。 valve_24hrs.csv：记录了暴露于9种实验盐度下24小时后的个体壳瓣开合观测记录，缺失值代表已潜穴且无法观测到壳瓣位置的个体。 valve_28hrs.csv：记录了暴露于9种实验盐度下28小时后的个体壳瓣开合观测记录，该观测在将标本重新放置为壳顶朝上后进行。 osm.csv：记录了暴露于9种实验盐度下28小时后的个体血淋巴渗透压（hemolymph osmolality）测量数据，渗透压单位为mOsm kg⁻¹。 th.csv：记录了暴露于9种实验盐度下28小时后的个体组织含水量测量数据，组织含水量以相对于组织湿重的水百分比表示。 lactato.csv：记录了暴露于9种实验盐度下28小时后的个体闭壳肌（adductor-muscle）样本中的乳酸浓度测量数据。 ldh.csv：记录了暴露于9种实验盐度下28小时后的个体闭壳肌（adductor-muscle）样本中的乳酸脱氢酶（lactate dehydrogenase, LDH）活性测量数据。 prot.csv：记录了闭壳肌（adductor-muscle）匀浆中的总蛋白浓度数据，该数据用于对乳酸脱氢酶活性进行标准化校正。 Bio-ORACLE v3海表盐度数据：用于表征各环境单元的背景盐度条件。 WorldClim 2.1生物气候数据：用于量化年降水量、最湿季度降水量以及降水季节性。 HydroRIVERS数据及衍生河口位置数据：用于量化河流径流量、流域特征以及物种分布区与河口之间的距离。