RNA Sequencing Analysis of Human Ovarian Cancer Cell Line OVCAR5 Treated with Stearic Acid, Oleic Acid, and CAY10566

This study investigates the anti-proliferative effects of long chain fatty acids on cancer cells, focusing on the specific impacts of oleate and stearate. We explored how these fatty acids, prevalent in biological systems, influence cellular processes. Our analysis revealed that the top 10 significantly upregulated Gene Ontology (GO) categories in OVCAR5 cells treated with 50 µM stearate (compared to control cells) are enriched in pathways related to the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress. Overall design: Our experimental design involved the treatment of OVCAR5 cells with various compounds for 24 hours. The treatments included: (i) DMSO (control), (ii) 1 µM CAY10566, (iii) 50 µM stearate with DMSO, (iv) 50 µM stearate with 1 µM CAY10566, (v) 50 µM oleate with DMSO, and (vi) 50 µM oleate with 1 µM CAY10566. After treatment, total RNA was extracted from each sample for RNA sequencing.