Unravelling the developmental roadmap towards human brown adipose tissue

We have developed and characterised functionally and molecularly a new chemically-defined protocol for the differentiation of human pluripotent stem cells (hPSCs) into brown adipocytes (BAs) that overcomes current limitations This protocol recapitulates step-by-step the physiological developmental path of human BAT. The BAs obtained expresses brown adipocyte and thermogenic markers, are insulin sensitive, and responsive to β-adrenergic stimuli. This new protocol is scalable, enabling the study of human BAs at early stages of development. mRNA profiles of the human embryonic stem celll line H9 and the human iPS cell line KOLF2-C1 at different stages of differentiation. Days of differentiation included D0, D2, D4. D8, D10, D12, D20 and D25 or D30. For H9 we have 3 biological replicates and for the KOLF2-C1, 5 biological replicates.