Transcriptome-wide mapping of RNA:protein interactions of PRPF8 and SNRNP200 (BRR2) with retinitis pigmentosa (RP) mutations by iCLIP

We used HeLa cells that express GFP-tagged PRPF8 with and without the RP mutations F2314L and Y2334N at physiological levels as well as SNRNP200-GFP with and without the RP mutations S1087L and R1090L and performed iCLIP using anti-GFP antibodies. We also used RPE cells with and without the Y2334N mutation inserted by CRISPR/Cas9 and compared their binding pattern on snRNAs and pre-mRNAs. For each GFP-tagged protein, 3 biological replicates were subjected to the iCLIP procedure with UV-crosslinking at 254 nm. Non-crosslinked samples were used as controls.