Alignment and phylogenetic tree of section Habrostictis

ITS+LSU alignment and tree of section Habrostictis (Orbilia) including Orbilia multiserpentina. The phylogenetic tree was based on a Maximum Likelihood analysis from the combined ITS / LSU sequence alignment. Analyses were done on the Phylosuite v. 1.2.3 platform (Zhang et al. 2020). The alignment was performed with MAFFT v. 7 (Katoh & Standley 2013) and manually checked and trimmed. The ML phylogenies were inferred using IQ-TREE 2 (Minh et al. 2020) under the model automatically selected by IQ-TREE for 5 000 ultrafast (Minh et al. 2013) bootstraps. Bootstrap support values and Bayesian posterior probabilities are given at the nodes. Orbilia poitevinica and O. paravitalbae were used as outgroup. The novel taxon is indicated in bold. The scale bar on the tree indicates the expected number of changes per site本数据集涵盖隶属于Orbilia属的Habrostictis组（包含多蛇盘菌Orbilia multiserpentina）的ITS（Internal Transcribed Spacer，内转录间隔区）与LSU（Large Subunit ribosomal RNA，大亚基核糖体RNA）序列比对结果及系统发育树。本系统发育树基于联合ITS与LSU的序列比对结果，采用最大似然法构建。所有分析均在Phylosuite v.1.2.3平台（Zhang等，2020）上完成。序列比对使用MAFFT v.7软件（Katoh与Standley，2013）完成，并经人工校验与裁剪。最大似然系统发育树的推断使用IQ-TREE 2软件（Minh等，2020）完成，模型由IQ-TREE自动选择，同时开展5000次超快速自举（Minh等，2013）检验。各节点处标注有自举支持值与贝叶斯后验概率。本研究以Orbilia poitevinica与O. paravitalbae作为外类群。新分类群以粗体标注。树图上的标尺代表每位点的预期替换数。