An integrated flow cytometry-based platform for isolation and molecular characterization of circulating tumor single cells and clusters. An integrated flow cytometry-based platform for isolation and molecular characterization of circulating tumor single cells and clusters

We demonstrate the feasibility of performing single cell RNA sequencing on cells isolated from whole blood using a novel flow cytometry-based platform Overall design: Whole transcriptome sequencing of single and pooled cells from a mouse pancreatic cancer cell line to validate rare cell pre-enrichment and isolation approach