Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration

# Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration There are data for 5 animal groups. Two groups of mice were presented with stimulus novelty or contextual novelty. Different two groups of mice were presented with stimulus novelty and dopamine neurons that project to the tail of the striatum (TS) were ablated or sham surgery was operated. A different group of mice were presented with stimulus novelty and dopamine activity in TS was recorded. All mice were recorded in videos and location of body parts was tracked with DeepLabCut. Some videos were also analyzed with Moseq. ## Description of the data and file structure # Data structure DLC (DeepLabCut) data is stored in .mat files. Given .mat files were named based on the following file structure: . +-- group1_name | +-- animal1_name | +-- session1_name | -- DLClabel.mat | +-- session2_name | +-- session3_name | +-- animal2_name | +-- animal3_name | -- bout.mat | -- bout_multi.mat +-- group2_name +-- group3_name \-- akiti_miceID_231129.xlsx There is a separate .mat file for each session. DLClabel.mat contains the variable "Labels" which has the following format: Labels(:,2) Nose x (pixel) Labels(:,3) Nose y (pixel) Labels(:,5) Leftear x (pixel) Labels(:,6) Leftear y (pixel) Labels(:,8) Rightear x (pixel) Labels(:,9) Rightear y (pixel) Labels(:,11) Tailbase x (pixel) Labels(:,12) Tailbase y (pixel) Labels(:,14) Tailmidpoint x (pixel) Labels(:,15) Tailmidpoint y (pixel) Labels(:,17) Tailtip x (pixel) Labels(:,18) Tailtip y (pixel) Labels(:,20) Head x (pixel) 'average of nose, leftear and rightear' Labels(:,21) Head y (pixel) Labels(:,22) Body x (pixel) 'average of head and tail base' Labels(:,23) Body y (pixel) Labels(:,24) Tail x (pixel) 'average of tailtip, midpoint and base' Labels(:,25) Tail y (pixel) Labels(:,26) head speed (pixel) Labels(:,27) head accerelation (pixel) Labels(:,28) head jerk (pixel) Labels(:,29) body speed (pixel) Labels(:,30) body accerelation (pixel) Labels(:,31) body jerk (pixel) Labels(:,32) nose distance from object (pixel) Labels(:,33) head distance from object (pixel) Labels(:,34) tailbase distance from object (pixel) Labels(:,35) body length (pixel) Labels(:,36) head speed related to object (pixel) Labels(:,37) head speed unrelated to object (pixel) Labels(:,38) tail-base from wall (pixel) MoSeq data is stored in `MoSeq_MiceIndex_wLabels_combine3L.mat`. This file contains the variable "Mice" which has the following format: Mice.name: mouse name Mice.novelty: stimulus novelty ‘S’ or contextual novelty ‘C’ Mice.ExpDay: experiment date Photometry data (Dopamine sensor in the tail of the striatum, TS) is stored in files named ` *_approach_start.mat`, `*_retreat.mat`, and `*_retreat_end.mat` (3 files for the first day of novelty for each animal). Original videos are stored in files named ‘_rgb.mp4’. Information about each mouse is stored in akiti_miceID_231129.xlsx. There are two types of sessions: · "novel"1, 2, 3, ... : an object was presented. 1, 2, 3 indicates day. For example, novel1 means that the data was obtained on the first day of object presentation. A same object was presented on following days. · "hab"1, 2 : habituation sessions with no object, before novelty day 1. There are 5 groups of mice: 1. "FP_all": recording of dopamine sensor signals in TS on novelty day 1 2. "stimulus": a novel object was presented (a different object was presented before habituation day 1 in a home cage) 3. "contextual": an unexpected familiar object was presented (a same object was presented before habituation day 1 in a home cage) 4. “saline”: sham surgery (vehicle injection), a novel object was presented 5. “6OHDA”: TS-projecting dopamine neurons were ablated, a novel object was presented MoSeq data was obtained using data of groups 2 to 5 on novelty day 1.Sharing/Access information ## Code/Software Matlab code files using this dataset are available on GitHub (

# 新物体探索任务中纹状体尾部（tail of the striatum, TS）多巴胺活动、DeepLabCut及MoSeq相关数据集
本数据集包含5组小鼠的实验数据。其中两组小鼠分别接受刺激新奇性（stimulus novelty）或情境新奇性（contextual novelty）刺激；另有两组小鼠在接受刺激新奇性刺激的同时，对投射至纹状体尾部的多巴胺神经元进行了消融处理或接受假手术；剩余一组小鼠在接受刺激新奇性刺激的同时，记录了其TS脑区的多巴胺活动。所有小鼠均录制了行为视频，并通过DeepLabCut追踪身体部位坐标。部分视频同时使用MoSeq进行分析。

## 数据与文件结构说明
DeepLabCut（简称DLC）数据存储为.mat格式文件，文件命名遵循如下层级结构：
根目录下按组别分为多个子文件夹，每个组别文件夹下包含对应小鼠的子文件夹；每个小鼠文件夹下包含多个实验会话子文件夹，其中部分会话文件夹内存在`DLClabel.mat`文件。其余小鼠文件夹下则包含`bout.mat`与`bout_multi.mat`文件。根目录下同时存在`akiti_miceID_231129.xlsx`文件。

每个实验会话对应一个独立的.mat文件。`DLClabel.mat`中包含变量`Labels`，其数据格式如下：
Labels(:,2)：鼻尖x轴像素坐标
Labels(:,3)：鼻尖y轴像素坐标
Labels(:,5)：左耳x轴像素坐标
Labels(:,6)：左耳y轴像素坐标
Labels(:,8)：右耳x轴像素坐标
Labels(:,9)：右耳y轴像素坐标
Labels(:,11)：尾基x轴像素坐标
Labels(:,12)：尾基y轴像素坐标
Labels(:,14)：尾中点x轴像素坐标
Labels(:,15)：尾中点y轴像素坐标
Labels(:,17)：尾尖x轴像素坐标
Labels(:,18)：尾尖y轴像素坐标
Labels(:,20)：头部x轴像素坐标（鼻尖、左耳、右耳的平均值）
Labels(:,21)：头部y轴像素坐标
Labels(:,22)：躯干x轴像素坐标（头部与尾基的平均值）
Labels(:,23)：躯干y轴像素坐标
Labels(:,24)：尾部x轴像素坐标（尾尖、尾中点与尾基的平均值）
Labels(:,25)：尾部y轴像素坐标
Labels(:,26)：头部运动速度（像素）
Labels(:,27)：头部运动加速度（像素）
Labels(:,28)：头部运动加加速度（像素）
Labels(:,29)：躯干运动速度（像素）
Labels(:,30)：躯干运动加速度（像素）
Labels(:,31)：躯干运动加加速度（像素）
Labels(:,32)：鼻尖与物体的距离（像素）
Labels(:,33)：头部与物体的距离（像素）
Labels(:,34)：尾基与物体的距离（像素）
Labels(:,35)：躯干长度（像素）
Labels(:,36)：与物体相关的头部运动速度（像素）
Labels(:,37)：与物体无关的头部运动速度（像素）
Labels(:,38)：尾基与墙壁的距离（像素）

MoSeq数据存储于`MoSeq_MiceIndex_wLabels_combine3L.mat`文件中，该文件包含变量`Mice`，其数据格式如下：
Mice.name：小鼠名称
Mice.novelty：新奇性类型，取值为`S`（刺激新奇性）或`C`（情境新奇性）
Mice.ExpDay：实验日期

纹状体尾部多巴胺传感器的荧光光度数据存储于以下命名格式的文件中：`*_approach_start.mat`、`*_retreat.mat`及`*_retreat_end.mat`（每只小鼠新奇性实验首日的数据包含上述3个文件）。

原始行为视频存储于`_rgb.mp4`格式文件中。

每只小鼠的相关信息存储于`akiti_miceID_231129.xlsx`文件中。

实验会话分为两类：
1. `novel1/2/3/…`：呈现新物体的测试会话，数字代表实验天数。例如`novel1`代表新物体呈现首日的数据，后续天数将呈现同一物体。
2. `hab1/2`：习惯化会话，在新奇性实验首日之前进行，未呈现物体。

本数据集共包含5组小鼠：
1. `FP_all`组：在新奇性实验首日记录TS脑区的多巴胺传感器信号
2. `stimulus`组：呈现新物体（在笼内习惯化首日之前，会呈现不同的物体）
3. `contextual`组：呈现意料之外的熟悉物体（在笼内习惯化首日之前，会呈现同一物体）
4. `saline`组：接受假手术（载体注射），并呈现新物体
5. `6OHDA`组：消融投射至TS脑区的多巴胺神经元，并呈现新物体

MoSeq数据仅使用第2至第5组小鼠在新奇性实验首日的数据。

## 共享与获取信息
## 代码与软件
使用本数据集的Matlab代码文件可于GitHub平台获取（