Supplementary figures and table for: Autologous split-thickness skin graft–acellular dermal matrix composite transplantation promotes physiologic skin remodeling: A paired, controlled study in rats

This dataset contains the 11 supplementary figures and 1 supplementary table accompanying the above-named article. The study tested the hypothesis that compositing an ultrathin (0.2-mm) autologous split-thickness skin graft (STSG) with human acellular dermal matrix (ADM) restores a dermal scaffold beneath the graft and thereby reduces wound contraction and promotes physiologic, rather than fibrotic, skin remodeling. Data were generated in a within-animal paired model: each of 20 male Sprague-Dawley rats received two dorsal full-thickness skin defects randomized to STSG alone or STSG+ADM, and wounds were followed on days 3, 7, 14, 21, and 28. The files comprise representative source images underlying the quantitative analyses reported in the article: Supplementary figures 1-2 document the surgical model and the modified thickness-calibrated dermatome; Supplementary figure 3 shows hematoxylin-eosin histology of normal skin and harvested grafts; Supplementary figure 4 shows the gross appearance of grafted wounds over time; Supplementary figure 5 shows body weight curves (mean ± SD) as a welfare/toxicity control; Supplementary figures 6-9 show, respectively, hematoxylin-eosin histology, collagen remodeling (Masson trichrome and Picrosirius red under polarized light, in which yellow-orange birefringence indicates type I and green indicates type III collagen), CD34 immunostaining of angiogenesis, and cytokeratin 14 immunofluorescence of re-epithelialization; Supplementary figure 10 shows scanning electron microscopy of the ADM architecture; and Supplementary figure 11 shows rat epidermal keratinocytes cultured on ADM, including CCK-8 proliferation data. Supplementary table 1 lists the primer sequences used for reverse transcription–quantitative PCR. These data support the article's main findings: compared with STSG alone, the composite graft reduced wound contraction by 19-43 percentage points, preserved graft thickness, and maintained total collagen deposition statistically indistinguishable from unwounded skin during peak remodeling. The images are provided for transparency and reviewer reference; quantitative endpoints derived from them (wound area, contraction rate, graft thickness, collagen area and type I/III ratio, CD34+ vessel density, CK14+ epithelial area, and gene expression) are reported in the article text, figures, and tables. Researchers may reuse these images as reference material for comparable grafting and dermal-substitution studies; Picrosirius red images were uniformly color-adjusted as disclosed in the article's figure legends.本数据集包含伴随上述署名文章的11幅补充图与1张补充表。本研究旨在验证下述假说：将超薄（0.2mm）自体薄层皮片（split-thickness skin graft, STSG）与人源脱细胞真皮基质（acellular dermal matrix, ADM）复合，可在皮片下方重建真皮支架，进而减少创面收缩，促进生理性而非纤维化的皮肤重塑。实验采用动物体内配对模型：20只雄性斯普拉格-道利（Sprague-Dawley）大鼠均接受两处背部全层皮肤缺损造模，随机分为单纯STSG组与STSG+ADM复合组，分别于术后第3、7、14、21、28天对创面进行随访观测。 本数据集所收录的文件为文章定量分析所依托的代表性原始图像：补充图1至2记录了手术造模模型与经过改良的厚度校准式取皮刀；补充图3展示正常皮肤与采集皮片的苏木精-伊红（hematoxylin-eosin）组织学染色结果；补充图4展示不同时间点移植创面的大体外观；补充图5展示体重变化曲线（均值±标准差），作为福利与毒性对照；补充图6至9分别展示苏木精-伊红组织学染色、胶原重塑情况（Masson三色染色与偏振光下的天狼星红（Picrosirius red）染色，其中黄橙色双折射代表I型胶原，绿色代表III型胶原）、血管新生的CD34免疫染色，以及再上皮化过程中的细胞角蛋白14（cytokeratin 14）免疫荧光染色；补充图10展示脱细胞真皮基质结构的扫描电子显微镜（scanning electron microscopy）图像；补充图11展示在脱细胞真皮基质上培养的大鼠表皮角质形成细胞，包含CCK-8增殖实验数据。补充表1列出了逆转录定量PCR（reverse transcription–quantitative PCR）所用的引物序列。 本数据集的数据支撑了文章的核心研究结论：与单纯STSG组相比，复合皮片可使创面收缩率降低19至43个百分点，保留移植皮片厚度，且在重塑高峰期的总胶原沉积量与未受损皮肤无统计学差异。本次公开图像旨在提升研究透明度并供审稿人参考；从图像中提取的定量终点包括创面面积、收缩率、移植皮片厚度、胶原面积与I/III型胶原比值、CD34+血管密度、CK14+上皮面积以及基因表达水平，上述指标均已在文章正文、图表中进行了报道。研究人员可将本数据集的图像作为同类移植与真皮替代研究的参考资料进行复用；天狼星红染色图像已按照文章图例中说明的方式进行了统一色彩调整。