File S1 - Epigenetic Silencing of miR-338-3p Contributes to Tumorigenicity in Gastric Cancer by Targeting SSX2IP

Supporting information figures. Figure S1. miR-338-3p inhibits GES-1 proliferation and induces apoptosis, but has no influence on migration. (A) GES-1 cells proliferation were performed by the WST assay. GES-1 cells were transfected with miR-338-3p mimics, NC mimics, anti-miR-338-3p or anti-NC at a final concentration of 100 nM.The WST assay was performed every 24 h for 4 days. The results are means of three independent experiments ± S.D.(*PPPFigure S2. Inhibition of miR-338-3p induces the proliferation of SGC-7901 cells. (A) SGC-7901 cells proliferation were performed by the WST assay. SGC-7901 cells were transfected with anti-miR-338-3p or anti-NC at a final concentration of 100 nM.The WST assay was performed every 24 h for 4 days. The results are means of three independent experiments ± S.D.(*PPFigure S3. Inhibition of miR-338-3p induces migration and invasion of SGC-7901 cells. (A) Representative photographs of migratory cells on the membrane (magnification 100×).(B) Average migratory cell number of three independent experiments ± S.D. (*PPFigure S4. Inhibition of miR-338-3p inhibits apoptosis of SGC-7901 cells. (A–B) Representative histograms depicting apoptosis of SGC-7901 cells transiently transfected with anti-miR-338-3p or anti-NC. Cells were stained with PI and Annexin V-FITC at 48 h post-transfection. (C) The percentage of apoptotic cells of three independent experiments ± S.D. are shown (*PFigure S5. SSX2IP has no influence on the expression of miR-338-3p. Both SGC-7901 cells and GES-1 cells were transiently transfected with GFP-SSX2IP, GFP-Vector control, siRNA-SSX2IP or siRNA-NC. Relative expressions of miR-338-3p in these cell lines were detected by qPCR. (DOC)