Enhanced genome annotation strategy provides novel insights on the phylogeny of 'Flaviviridae': Supplementary material

SUPPLEMENTARY MATERIAL <strong>Index</strong> Table S1 (tableS1.csv): genomic data. Table S2 (tableS2.csv): character categorization for selected nodes. Table S3 (tableS3.csv): programs and parameters. Table S4 (tableS4.csv): annotation efficiency. File S1 (fileS1.gff): gene annotation. File S2 (fileS2.xml): configuration file for BEAST 2 (configuration.xml). Figure S1 (figureS1.pdf): dendrogram depicting the hierarchical clusters of trees based on match-split distances. Figure S2 (figureS2.pdf): full version of the working phylogenetic hypothesis (tree No. 0 in table 1). <strong>Figure captions</strong> Figure S1: A dendrogram depicting the hierarchical clusters of trees based on match-split distances. Outgroup sequences (<em>Hepacivirus</em>, <em>Pegivirus</em>, and <em>Pestivirus</em>) were removed to guarantee the compared tree topologies would have the same terminals. Tree numbers correspond to those in table 1 of the manuscript. I. No outgroup sequences; some matrices were partitioned. II. Outgroup sequences and partitioned matrices. *This tree was produced without outgroup sequences. Figure S2: Full version of the working phylogenetic hypothesis (tree No. 0 in table 1). Branch lengths represent an estimation of the number of substitutions per site. Node labels indicate SH-aLRT support / ultrafast bootstrap (only shown if one of there is a value below 90%). Clade names correlate to the character categorization analysis (see table S2). Branch labels represent the four genera: I = <em>Pestivirus</em>; II = <em>Pegivirus</em>; III = <em>Hepacivirus</em>; IV = <em>Flavivirus</em>. * The Ecuador Paraiso Escondido virus (EPEV) was isolated from sand flies (<em>Psathyromyia abonnenci</em>). The EPEV was the first sand fly-borne <em>Flavivirus</em> identified in the New World. <strong>Manuscript title</strong> FLAVi: an enhanced annotator for viral genomes of <em>Flaviviridae</em>. <strong>Authors</strong> de Bernardi Schneider, Adriano. University of California San Diego. ORCID: 0000-0001-7487-266X. Jacob Machado, Denis. University of North Carolina at Charlotte. ORCID: 0000-0001-9858-4515. Corresponding author. Guirales, Sayal. University of North Carolina at Charlotte. Janies, Daniel. University of North Carolina at Charlotte. <em>First author</em>: Adriano de Bernardi Schneider and Denis Jacob Machado have contributed equally to the manuscript. <strong>Contact information</strong> Corresponding author: Denis Jacob Machado, Ph.D. OrcID: 0000-0001-9858-4515. Email: dmachado [at] uncc.edu. <strong>Other additional material</strong> In addition to the material listed above, all 31 tree topologies and 15 alignment matrices discussed in this manuscript will are available in TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S24096) after the publication of the manuscript. The FLAVi pipeline and all the original scripts are available at GitLab (https://gitlab.com/MachadoDJ/FLAVi). The web application can be accessed at http://flavi-web.com.