Quantitative Fitness Profiling of three deletion collections. Saccharomyces cerevisiae

Genetic background affects gene-environment and gene-drug interactions. We compared using the auxotrophic YKO collection as a benchmark, two prototrophic deletion collections created using different methods (backcrossing vs plasmid complementation). We found collection-specific and condition-specific biology and caution users to carefully consider the advantages and disadvantages of each collection for each biological question of interest.