Automatic Blood Protein Enrichment by Magnetic-COF Polymers

Blood proteome is
a highly informative biological fluid,
reflecting
physiological and pathological states across the entire body. It contains
thousands of proteins spanning a dynamic range of more than 10 orders
of magnitude. This makes blood proteome an ideal matrix for disease
diagnosis, prognosis, therapeutic selection, and monitoring. However,
comprehensive and reproducible analysis of blood proteins remains
technically challenging, primarily due to the overwhelming presence
of high-abundance proteins that obscure low-abundance targets. Blood
proteome analysis offers several advantages over tissue-based diagnostics:
it enables real time, longitudinal sampling, captures systemic physiological
or pathological changes, and provides access to tissue-derived proteins.
Automation offers consistent handling, reduced human error, and better
reproducibility, which are essential for translating proteomics into
routine biomedical workflows. We designed this platform to integrate
Magnetic-COF-based protein capture with scalable robotic handling,
enabling blood protein enrichment and LC-MS/MS analysis, resulting
in more than 4000 plasma proteins identified with a throughput of
30 samples per day. By combining molecular selectivity, magnetic enrichment,
and automation, our strategy addresses key limitations in current
blood proteomics workflows and offers a flexible foundation for the
research and discovery of proteomics from pancreatic ductal adenocarcinoma
patients.