A tagged histone variant exploits cancer-specific pathway in human cells

Little is knownThe use of tags is ubiquitous in scientific research, has allowed researchers the capability to make great strides in scientific discoveries that have left lasting impact in many disciplines. Here, we investigate the aberrant functional roles associated with C-terminally TAP-tagged CENP-A (CpA-TAP) when compared to centromeric native CENP-A (CpA). ChIPseq of native CpA or CpA-TAP in control or transfected HeLa cells, in a DAXX knock-out (KO) cell line, and double DAXX KO + HIRA knock-down. ChIPseq was replicated for GFP-CpA expressing HeLa cells.