Tip60-mediated H2A.Z acetylation is required for neuronal fate specification and bivalent gene activation [Cut & Tag]

Tip60-dependnet histone modification/occupancy dynamics during Ascl1-mediated direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced neuronal cells. Overall design: Chromatin occupancy dynamics of H2A.Z, H2A.Zac and Tip60 was determined using Cut&Tag (Kaya-Okur et al., 2019) at different times of MEF-iN cell reprogramming. The impact of Tip60 depletion was assessed in rtTA control or Ascl1-transduced (i) MEFs co-expressing shTip60 or shNTC (for H2A.Z and H2A.Zac), and (ii) Tip60 conditional knockout (cKO) MEFs expressing Cre or DCre control (for H3K4me3).