Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer

PBMCs from 4 donors were stained with the influenza A virus HLA-A24/PB1 498-505 tetramer. Two donors (non-LIFT8 and non-LIFT12) showed TCR independent KIR binding to tetramers whereas the other two donors (Non-LIFT14 and Non-LIFT10) showed TCR specific binding. Cells were single-cell sorted on a BD Aria III into 96-well plates containing 0.5 µl dNTP mix (10 mM), 0.5 µl oligo-dT pri- mer (5 µM), and 1 µl lysis buffer (prepared by adding 1 µl RNase inhibitor to 19 µl Triton X-100 solution, 0.2% v/v).