Infrared nano-spectroscopy of single cell sections at the ultrastructural level -- Dataset

This data set contains the underlying data of the publication “High-sensitivity infrared nano-spectroscopy of ultramicrotomed resin-embedded cell sections” by F. Marxer et al. s-SNOM microscopy data sets ---------------------------------------- This data set corresponds to the s-SNOM microscopy data acquired using the neaSNOM microscope (attocube Systems GmbH). The measured raw data is stored using Gwyddion Simple Field (GSF) open source format. The description could be found here: https://gwyddion.net/documentation/user-guide-en/gsf.html Each GSF file contains a single channel data. File names use specific suffixes to identify the specific channels, as outlined below: Optical Signal Channels (IR detector) …O0A raw.gsf: Near-field amplitude image (in millivolts) of the 0th demodulation order, forward direction …O0P raw.gsf: Near-field phase image (in radians) of the 0th demodulation order, forward direction …R-O0A raw.gsf: Near-field amplitude image (in millivolts) of the 0th demodulation order, backward direction …R-O0P raw.gsf: Near-field phase image (in radians) of the 0th demodulation order, backward direction Note: Additional demodulation orders from 1 through 5 follow the same naming convention, e.g. …O1A raw.gsf. Mechanical Signal Channels (AFM quadrant detector) …M0A raw.gsf: Amplitude image (in volts) of the 0th demodulation order, forward direction …M0P raw.gsf: Phase image (in radians) of the 0th demodulation order, forward direction …R-M0A raw.gsf: Amplitude image (in volts) of the 0th demodulation order, backward direction …R-M0P raw.gsf: Phase image (in radians) of the 0th demodulation order, backward direction Note: Additional demodulation orders from 1 through 5 follow the same naming convention, e.g. …M1A raw.gsf. Topography …Z raw.gsf: Topography of the sample in nanometers. nano-FTIR spectroscopy data sets -------------------------------------------- This data set corresponds to the nano-FTIR spectroscopic (Fourier-transform infrared nanospectroscopy) measurements acquired using the neaSNOM microscope (attocube System GmbH). All interferogram files are stored as plan-text and can be opened with any standard text editor. + File Structure The relevant nano-FTIR interferogram files are in the folder “nano-FTIR”. Each interferogram file is named with the suffix "Interferograms.txt". Each file contains a header and a tabular data with the following columns. Row: index column for hyperspectral data (not used in this study)Column: index column for hyperspectral data (not used in this study)Run: interferogram count for indexing multiple interferograms acquired at the same location Depth: pixel number within one interferogramM: position of the reference mirror (not used in this study)O0A: amplitude in V of the 0th demodulation order of the IR detector signalO0P: phase in radians of the 0th demodulation order of the IR detector signalO1A: amplitude in V of the 1st demodulation order of the IR detector signalO1P: phase in radians of the 1st demodulation order of the IR detector signalO2A: amplitude in V of the 2nd demodulation order of the IR detector signalO2P: phase in radians of the 2nd demodulation order of the IR detector signalO3A: amplitude in V of the 3rd demodulation order of the IR detector signalO3P: phase in radians of the 3rd demodulation order of the IR detector signalO4A: amplitude in V of the 4th demodulation order of the IR detector signalO4P: phase in radians of the 4th demodulation order of the IR detector signalO5A: amplitude in V of the 5th demodulation order of the IR detector signalO5P: phase in radians of the 5th demodulation order of the IR detector signal For help on how to read or interpret these data please refer to the corresponding authors of the paper.