Transcriptome profiling of Blimp-1-deficient B cells from distinct divisions during plasma cell differentiation

To examine the cell division that Blimp-1 functioned during B cell differentiation we adoptively transferred CellTrace Violet labeled Blimp-1-deficient or control B cells into congenically disparate uMT hosts. Following transfer, mice were innoculated with LPS to induce B cell differentiation and 72 hours later cells from distinct divisions were FACS sorted for RNA-seq Overall design: RNA-seq on cohorts of Blimp-1-deficient or control B cells from distinct divisions during in vivo B cell differentiation