RNAseq: H3K9 demethylase KDM3A potentiates interferon signaling and macrophage-driven inflammation in atherosclerosis

This repository contains raw and processed RNA sequencing (RNA-seq) data generated to characterize transcriptional differences between wild-type or Kdm3a-/- RAW264.7 macrophages and murine bone marrow derived macrophages, in presence and absence of lipopolysaccharide. RAW264.7 cells were maintained in DMEM supplemented with 2mM L-glutamine, penicillin (100U/mL), streptomycin (100U/mL) and 10% fetal calf serum. All supplements are from Gibco, Life Technologies. Cells were cultures in humidified atmosphere with 5% CO2 at 37C. For Bone marrow derived macrophages, femurs and tibia of C57BL/6 mice (wild-type or Kdm3a-/-) were flushed with ice-cold PBS, and cultured in RPMI-1640 supplemented with 2mM L-glutamine, penicillin (100U/mL), streptomycin (100U/mL) and 10% fetal calf serum, supplemented with 15% L929-conditioned medium for 8 days. Kdm3a-/- RAW264.7 cells were generated using CRISPR/Cas9, targeting exon 4 with a gRNA integrated in pX330(Addgene #42230). When stimulated, lipopolysaccharide (B5,Sigma) was provided at 100ng/mL for RAW264.7 and 10ng/mL for C57BL/6 bone marrow derived macrophages. These data include: single-end sequencing fastq.gz Tab-delimited count table Tab-delimited RPKM quantification table Metadata