Bacillus surface evolution

We studied the evolution of surface expansion in Bacillus subtilis and Bacillus cereus and used flow cytometry to determine the cell state composition (e.i., ) at both the colony edge and in the center.
Conclusion:
We observed that mutations in pleiotrophic hubs massively shifted the cell state composition of colonies at both the colony edge and center. These mutations affected the cell state composition both directly, by lowering the fraction of sporulating cells, and indirectly, by facilitating advanced colony spreading. We did various controls, which are not included among the files, including unstained samples or stained samples at distinct stages of liquid cell suspensions. To confirm accuracy of gating we previously also have performed imaging cytometry. These made it possible to optimize gating of cell types.