Insertion of oligomers into BBMV from resistant and susceptible larvae of P. gossypiella with 0.5 μg of Cry1Ac or Cry1AcMod.

Cry1Ac or Cry1AcMod activated toxins (0.5 μg) were incubated with BBMV (15 μg) from the different populations and the membrane pellets were recovered by centrifugation, separated by SDS-PAGE after three min heating at 50°C and revealed in western blot assays with anti-Cry1Ac antibody as described in Materials and Methods. Lanes 1 and 2 controls of BBMV without toxin from APHIS-S and AZP-R, respectively; lanes 5 and 8 are controls of soluble Cry1Ac and Cry1AcMod, respectively; lane 3, BBMV from APHIS-S precipitated after incubation with Cry1Ac; lane 4, BBMV from AZP-R precipitated after incubation with Cry1Ac; lane 6, BBMV from APHIS-S precipitated after incubation with Cry1AcMod; lane 7, BBMV from AZP-R precipitated after incubation with Cry1AcMod. Numbers under the 200 kDa bands represent the percentage of pixels relative to the 200 kDa band of APHIS-S BBMV with Cry1Ac toxin that corresponds to 100, calculated after scanning densitometry of the bands.