Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [indels]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Characterization of indels following Cas9-induced breaks in selected amplicons containing a SNP in the protospacer.