Whole-transcriptome analysis in breast cancer with or without lymph node metastasis

To identify the mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) involved in the pathogenesis of breast cancer, we performed the whole transcriptome sequencing. The whole transcriptome sequencing was performed with three breast cancer tissues with or without lymph node metastasis in each group, respectively. The results showed that 728 mRNAs, 131 lncRNAs, and 144 circRNAs were differentially expressed in lymph node metastasis group and no lymph node metastasis group (fold change=2, p<0.05). These data indicate that dysregulation of mRNAs, lncRNAs, and circRNAs may contribute to breast cancer progression. Overall design: We performed the whole-transcriptome sequencing in six cases of breast cancers, including three cases with lymph node metastasis(M) and three cases without lymph node metastasis(N), and identified the expression profiles of mRNAs, lncRNAs, and circRNAs.