Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021

Individuals were blood sampled from the jugular vein under general anaesthesia. Samples were collected into EDTA-coated Vacutainers before plasma and erythrocytes were separated by centrifugation then snap frozen in liquid nitrogen within 10 minutes of collection. Samples were shipped to the UK within 3 months under cryogenic conditions, and stored at -80C until analysis. Analyses were performed blindly with respect to sample identity, and all steps were conducted on ice. All chemicals were HPLC grade, and chemical solutions were prepared using Milli-Q water. Assays were conducted within one year of collection. Malondialdehyde was determined by HPLC with fluorescence detection (Agilent 1000). Protein carbonyls, glutathione and superoxide dismutase were determined using colorimetric assay kits (Cayman Chemical Company) and a Molecular Devices plate reader (Spectramax M2).

研究对象于全身麻醉状态下经颈静脉采集血液样本。样本采集于乙二胺四乙酸（EDTA）预处理的真空采血管（Vacutainer）中，经离心分离血浆与红细胞后，于样本采集后10分钟内置于液氮中速冻。样本在低温冷冻条件下于3个月内运抵英国，并于-80℃下储存直至分析。所有分析均采用盲法设计，不暴露样本身份，且所有实验步骤均在冰浴条件下进行。所有试剂均为高效液相色谱（High Performance Liquid Chromatography, HPLC）级，试剂溶液均使用密理博（Milli-Q）超纯水配制。所有检测均在样本采集后一年内完成。丙二醛（Malondialdehyde）采用高效液相色谱-荧光检测法测定，所用仪器为安捷伦（Agilent）1000型色谱仪。蛋白质羰基、谷胱甘肽与超氧化物歧化酶均采用比色法检测试剂盒（Cayman Chemical Company）及美谷分子（Molecular Devices）Spectramax M2型酶标仪完成检测。