An insight into the sialomes of Corethrella apendiculata and Lutzomyia umbratilis

Saliva of blood sucking arthropods contains a complex mixture of bioactive polypeptides that disarm their hosts blood coagulation, platelet aggregation and vasoconstriction. We here run barcoded cDNA from the salivary glands of adult female Corethrella appendiculata, a frog biting midge, and two populations of Lutzomyia umbratilis, on a single lane of a Hiseq Illumina sequencer. Sequences were assembled using Abyss and SoapdenovoTrans with several k values and the resulting contigs were further assembled by blast and cap3 iterations. There was some contamination between samples that were filtered out in the final results.