ChIP-seq in MEL cells. Mus musculus

Transcription factors (TFs) act as complexes with other co-factors to tune gene expression. Here a comprehensive analysis of the genome-wide occupancy of seven TFs highlights their properties during erythroid differentiation. We investigated the targets of the crucial erythroid TF GATA1 during erythroid differentiation in the context of a combinatorial network of LDB1, TAL1, FOG1, GFI1B, RUNX1 and p300. We analyzed the dynamics of the GATA1 complexes formed with either FOG1 or GFI1B and show that they control different processes essential for erythroid differentiation. GATA1/FOG1 and GATA1/GFI1B complexes preferentially bind repressive and activating regulatory elements respectively. In addition we show that RUNX1 and PU.1 repressed genes relevant for blood cell development, are targets of GATA1. On the basis of binding site analysis we also postulate the contribution of a number of additional TFs to the respective repressing or activating properties of GATA1/FOG1 or GATA1/GFI1B bound regulatory elements.