High-throughput RNA sequencing analysis of mouse peritoneal cells from wild type and Lactoferrin knockout mouse during Lipopolysaccharide(LPS) stimulation

Purpose: The goal of this study is using RNA sequencing analysis to evaluate the role of lactoferrin in the LPS-induced acute inflammation in mouse Methods: 4 hs after intraperitoneal injection with LPS(4mg/kg) or equal volume PBS , the peritoneal cells were isolated from the peritoneal lavage fluid from wild type and lactoferrin knockout mice (n=3 for each group) . Then the cell RNA was sequenced by the HiSeq 2500 high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. Conclusions: Our study describes the mRNA expression profile changes of peritoneal cells from wild type and lactoferrin knockout mice during LPS-induced acute inflammation The peritoneal cells were isolated from the wild type and lactoferrin mice, and cellular RNA was used for RNA-sequencing to show the gene expression profile change induced by lactoferrin deficiency during LPS-induced acute inflammation