Example of an alternatively spliced transcript (Tb11.02.2700).

(A) Display of the splicing profile for Tb11.02.2700 using Gbrowse. Spliced leader tags from bloodstream form (red) and procyclic form (green) are shown; numbers indicate tags per million (TPM). The position and size of the RT qPCR amplicon is depicted by a black bar (RT qPCR amplicon). Below the bar the mRNA abundance ratios as determined by SLT and qPCR respectively, are shown. (B) Expected size of PCR product using a spliced leader-specific oligonucleotide together with a gene specific oligonucleotide are depicted. Gel shows PCR products from procyclic cDNA.