five

Proximity labeling via MLF2-APEX2 fusion

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NIAID Data Ecosystem2026-03-13 收录
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https://www.omicsdi.org/dataset/pride/PXD036262
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Proximity labeling approach to identify protein inside nuclear envelope blebs arising in Torsin-deficient HeLa cells. WT and TorsinKO cells were engineered to express MLF2-APEX2 fusion. APEX reaction was carried out via 1 mM H2O2 for 1 minute in the presence of biotin phenol. Biotinylated protein were captured via streptavidin beads. Each APEX reaction was accompanied by an untreated (no H2O2) control.
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2022-08-25
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