Single-cell RNA-sequencing of wild-type, I830077J02Rik, C130074G19Rik and A530016L24Rik gene knockouts at day 7 of embryoid body differentiation

Haematopoietic and endothelial cells derive from a subset of Flk-1 expressing cells. In vitro differentiation of mouse embryonic stem cells (mESCs) to embryoid bodies (EBs) recapitulate several aspects of early embryonic development. We previously identified three candidate uncharacterized Riken genes (I830077J02Rik, C130074G19Rik and A530016L24Rik) potentially important for the cell lineage commitment toward haematopoietic precursors. Here, we investigate the transcriptomic profiles of cells depleted for these genes at day 7 of embryoid body differentiation, by scRNA-seq.