RNA-seq of patients with aplastic anemia. RNA-seq of patients with aplastic anemia in the presence or absence of levamisole in vitro

BM nucleated cells (BMNCs) from healthy donors and patients with NSAA at the time of initial diagnosis were separated, and then co-cultured with or without 40 μg/mL LMS for 48 hours in vitro. Cells were harvested and total RNA were extracted. The RNA-sequencing assay were carried out using the Illumina HiSeq X platform and the data obtained were analyzed by following the Hisat2 protocol