Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice

We examined the effect of chronic high fat diet (HFD) on amyloid deposition and cognition of 12-months old APP23 mice, and correlated the phenotype to brain transcriptome and lipidome. HFD significantly increased amyloid plaques and worsened cognitive performance compared to mice on normal diet (ND). Overall design: Male and female APP23 mice (mean age 11.7 months) were randomly assigned to ND (Prolab Isopro RMH 3000, Lab Diet) or HFD (D12079B RD Western Diet, Research Diets). HFD supplemented 40% calories from fat; 16.8% from protein and 42.6% from carbohydrates. Fat was provided as anhydrous milk fat and corn oil. The mice had free access to water and food. After 3 months of feeding, behavior was assessed at mean age of 14.7 months.