Sequences GB1-SEM1(86-107) and SEM1(86-107)

We attached nucleotide sequence of insertion GB1-SEM40 with his-tag (seq_gb1-sem40); amino acid sequences of GB1-SEM40 (file aa_seq_gb1-sem40) and SEM40 after TEV cleavage (file aa_seq_sem40_after_TEV); pETGB1::sem40 construction, annotated sequence (file petGB1-sem40); sequence of SEM40 amplificated from the Human genome, with restriction sites (NcoI, XhoI) and stabilizing TTTTTT tails (file sem40); fragment amplificated with T7-primers from pETGB1::sem40, It contains T7 promotor, GB1-SEM40 coding sequence, and T7 terminator (file T7sem40).

本数据集附带如下内容：带有组氨酸标签（his-tag）的插入片段GB1-SEM40的核苷酸序列（文件seq_gb1-sem40）；GB1-SEM40以及经烟草蚀刻病毒（TEV）蛋白酶切割后的SEM40的氨基酸序列，分别对应文件aa_seq_gb1-sem40与aa_seq_sem40_after_TEV；重组质粒pETGB1::sem40的构建信息与注释序列（文件petGB1-sem40）；从人类基因组中扩增得到的、带有限制性酶切位点（NcoI、XhoI）及稳定TTTTTT尾的SEM40序列（文件sem40）；以T7引物从重组质粒pETGB1::sem40中扩增得到的片段，该片段包含T7启动子、GB1-SEM40编码序列与T7终止子（文件T7sem40）。