Human 293T cells lysosomal osmotic stress response
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.omicsdi.org/dataset/pride/PXD047404
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The goal of this project is to identify changes of the lysosomal surface proteome upon the induction of lysosomal vacuolation. 293T cells stably expressing LAMP1-GFP-TurboID or Lyso-TurboID were treated with a small molecule apilimod or DMSO to trigger lysosomal vacuolation, after which the lysosomal surface proteins were labeled with biotin by TurboID. The biotinylated proteins by Lyso-TurboID were purified by streptavidin beads for mass spectrometry.
创建时间:
2025-08-21



