Text S1 - Modeling Mitochondrial Bioenergetics with Integrated Volume Dynamics

The supplemental material consists of three parts. Part S1 lists the state variables comprising the model, updated Gibbs free energy of formation values, additional and revised dissociation constants, temperature correction method, and general model parameters. Part S2 introduces of the set of 60 non-linear ODEs, five algebraic conservation expressions (for ATP, GTP, NADH, UQH2 and c2+), five matrix cation ODEs (for H+, K+, Na+, Mg2+ and Ca2+) and the algebraic expressions for computing matrix and intermembrane space (IMS) water volumes and matrix Cl− is presented. Part S3 discusses the model rate equation derivations and provides all the associated parameter definitions and values.
(2.73 MB DOC)