Cohesin cofactor dosage sets the rate of loop extrusion, rendering genome folding tunable yet vulnerable to genetic disruption

The following files corresponds to the raw microscopy images used in the iFRAP experiment on RAD21 for the article named "Cohesin cofactor dosage sets the rate of loop extrusion, rendering genome folding tunable yet vulnerable to genetic disruption".

Briefly, the iFRAP experiments were done by imaging RAD21-HALO proteins. The labeling was done by adding 100nM of HALO-JF-549 ligand to the media for 30 min then washed three times with PBS before imaging. The images were acquired on a spinning disk confocal microscope and saved as .tif files. The filenames are composed as following: date_FRAP_tagged-prot_depletion_laser-power_LP_microscope-settings.tif.

The exact matching between filename and experimental conditions can be found in the dataframe "combined_dataframe.csv" in which you can find the columns:

-mean_list_interp_bleached: "mean intensity in the bleached region of the nuclei"
-mean_list_interp_unbleached: "mean intensity in the unbleached region of the nuclei"
-background_int: "mean intensity in a region of the image containing no cells"
-unfrap_cell: "mean intensity of nuclei that were not FRAPped during the experiment"
-nucleus: "The nucleus id within the movie"
-frame: "frame number"
-tagged_prot: "protein targeted by the degron"
-depletion: "depletion condition, WT=untreated"
-filename: "the complete filename"

The exact information about the processing and more detailed information about the data can be found in the github: "https://nesslfy.github.io/cohesin_residence_time/index.html".
The corresponding code to analyze these data can be found: https://github.com/NessLfy/cohesin_residence_time/blob/master/notebooks/analysis_FRAP_data.ipynb