Single embryo analysis of monozygotic twin blastocysts of the mouse respecting pair associations. Mus musculus

Upon 2-cell embryo splitting, individual blastomeres were cultured in KSOM(aa) medium and developed into blastocysts, which were compared and contrasted with each other respecting pair associations (e.g. twin vs cotwin) Transcriptome analysis revelaled minimal differences between twins and unmanipulated controls, in contrast to larger differences between twins and cotwins; these differences are mainly related to the epiblast lineage Overall design: Pair association (e.g. twin and cotwin from the same 2-cell embryo) was rigorously observed