EMT indeced by TGF-beta1

Pulmonary fibrosisinvolves the process known as epithelial-to-mesenchymal transition (EMT) in which alveolar epithelial cells become mesenchymal cells, as well as lung fibroblast changes to abnormal myofibroblasts, and the deposition of extracellular matrix molecules such as collagen. These changes are irreversible, so current treatment aims to halt disease progression. Pulmonary fibrosis can be drug-induced or caused by an infectious disease or occupational dust inhalation, but the cause is unknown in idiopathic pulmonary fibrosis (IPF). This project analyzes gene expression during EMT or fibrosis, using EMTmodel induced in human alveolar epithelial cells (A549) by transforming growth factor (TGF)-b1 stimulation or pulmonary fibrosis model mouse.