Discovery of a novel polymer for xeno-free, long-term culture of human pluripotent stem cell expansion

Discovery of poly(TCDMDA-blend-BA) polymer substrate offers a cost-effective and xeno-free adherent culture system for human pluripotent stem cells (hPSCs) in E8 medium. This is a scalable substrate which can be readily applied to common tissue culture plasticware (TCP), whilst retaining stem cell integrity after long-term serial passage. Fulfillment of all regulatory cell culture requirements makes this a desirable expansion platform for clinical-scale, therapeutic and biomedical applications. Data presented herein includes fluorescence microscopy of high-throughput attachment screening studies.