EdU-Click on Gut Bacterial Isolates

Flow cytometry data on bacterial isolates oringating from the human gut, incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, in a 1:1 ratio of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating cells.

The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.

In the file names:

AM = Akkermansia muciniphila

BC = Bacteroides caccae

BLI = Bifidobacterium longum subsp. infantis

BF = Bacteroides fragilis

CB = Clostridium beijerinckii

EC = Escherichia coli K12

EF = Enterococcus faecalis

PI = Prevotella intermedia

RB = Ruminococcus bromii