EdU-Click on Gut Bacterial Isolates

Flow cytometry data on bacterial isolates oringating from the human gut, incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, in a 1:1 ratio of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating cells. The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria. In the file names: AM = Akkermansia muciniphila BC = Bacteroides caccae BLI = Bifidobacterium longum subsp. infantis BF = Bacteroides fragilis CB = Clostridium beijerinckii EC = Escherichia coli K12 EF = Enterococcus faecalis PI = Prevotella intermedia RB = Ruminococcus bromii