Forskolin induced pluripotency of caudal fin fibroblasts of Topmouth culter.

As the passage number increases, the spontaneous aggregation phenomenon of TCCF2022 cells gradually weakens and eventually disappears, and occasional instances of post-passage cells being unable to proliferate are observed. This suggests that the characteristics of the cells may have changed during the culture process.We found that when individual factors were used to induce TCCF2022, on the third day after treatment with 5μM Forskolin, TCCF2022 cells regained the ability to form cell aggregates, and alkaline phosphatase detection was also positive. Therefore, we need to determine the possible mechanism of this state recovery through RNA-seq. The caudal fin fibroblasts of Topmouth culter with 5μm of Forskolin and without any treatment were cultured for seven days, and the RNA-seq data were analyzed for differential expression.