Additional file 2: Figure S1. of LncRNA HOTAIR promotes cell migration and invasion by regulating MKL1 via inhibition miR206 expression in HeLa cells

The effects on migration and invasion of MKL1 KO cells lines via CRISPR/Cas 9 system. (A). The MKL1 expression level was determined by western blot in MKL1 KO cells. GAPDH serves as the loading control. (B). Wound healing assay the effect on cell migration was determined after MKL1 depletion. (C). Quantification of the wound healing assay. (D). The effect on cell invasion was determined after MKL1 depletion in a Boyden chamber assay. (E). The number of cells on the underside of the filter was determined and significantly (P < 0.05) changed invasion is indicated. Data are presented as means ± S.D. and results are from one representative experiment of at least three. *, P < 0.05; **, P < 0.01 (Student’s t test). (ZIP 4524 kb)