Triplex assays of human genomic dsDNA (purified from saliva) for <i>CFTR</i> 2789+5G→A (1 bp T–G mismatch).
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The target was human genomic dsDNA, wild-type for CFTR. The 25-mer probes were 2789+5G→A-WT25C (wild-type) and 2789+5G→A-MUT25C (mutant). 500 nM YOYO-1 was present in each sample. TAF indicates Triplex-Associated Fluorescence.
创建时间：
2007-03-21
