Phototropic bending in auxin transport mutants of Arabidopsis.

Homozygous dark-acclimated seedlings were analyzed for phototropic bending induced by 8 h directional blue light (1 µmol m−2 s−1). Results represent the mean ± SD, n = 10 seedlings in three independent experiments except for pin1–1 and pin1–1 b1–1 b19–1, where eight and five seedlings were used, respectively, in each experiment. For assays of pin1–1 and pin1–1 b1–1 b19–1, progeny of a single plant heterozygous for pin1–1 were used in the bending assays. Reduced-bending pin1–1 seedlings and increased-bending pin1–1 b1–1 b19–1 seedlings were selected and grown to maturity then examined for pin-formed inflorescences to determine if they were homozygous for pin1. Significant differences compared to wild type (Col-0) are indicated. ND, not determined.