Characterization of gene profile between ALDH high LNCaP cells and ALDH low LNCaP cells. Characterization of gene profile between ALDH high LNCaP cells and ALDH low LNCaP cells

Acquisition of prostate cancer stem cells (PCSCs) manifested during androgen ablation therapy (ABT) contributes to castration-resistant prostate cancer (CRPC). However, little is known about the specific metabolites critically orchestrating this process. Here, we show t+B13hat IMPA1-derived inositol enriched in PCSCs is a key metabolite crucially maintaining PCSCs for CRPC progression and ABT resistance. Overall design: For fluorescence-activated cell sorting, cells were labeled with ALDEFLUOR Kit and sorted using MoFlo Astrios Cell Sorter (BECKMAN COULTER) in two ways including highest 10 % ALDH positive cells (ALDHhigh) and the lowest 10% ALDH negative cells (ALDHlow/-), following by cell culture and immunoblotting.