RNA sequencing (RNA-seq) of LAIR-1 overexpression in SKOV-3 ovarian cancer cell lines

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1), is widely expressed by solid tumor cells and functions as a tumor suppressor. Our previous studies have found that LAIR-1 is expressed in ovarian cancer tumor tissues and in a variety of epithelial ovarian cancer cell lines and that LAIR-1 could inhibit ovarian cancer cell growth by suppressing the PI3K-AKT-mTOR pathway. The aim of the present study was to obtain a comprehensive understanding of the molecular mechanism of LAIR-1 in regulating the biological behavior of ovarian cancer cells. The approach taken was to perform RNA-seq sequencing on SKOV3 cells overexpressing LAIR-1, and to conduct further validation by combinatorial integration of bioinformatics analyses to assess the genetic alterations and using biological experimental findings. There are two groups cells used for this expression profile comparison : NC (negative control) and OE(LAIR-1–overexpression) . Total RNA was extracted with Trizol, 1 μg of total RNA was hybridized to the probe and digested with RNase H followed by DNase I. RNA was purified using VAHTS RNA purification beads, 18.5 μL Frag/Prime Buffer (1x) was added, mixed by pipetting, and allowed to stand for 2 minutes at room temperature. After standing on a magnetic stand for 5 minutes to allow the solution to clear, 16 μL of supernatant was gently aspirated into a new nuclease-free centrifuge tube, and the samples were fragmented using a PCR instrument and sequenced using an Illumina sequencer.