Genome Skimming is a low-cost and robust strategy to assemble complete mitogenomes from ethanol preserved specimens

This project proposes a combination of methods as an excellent solution to obtain low cost/large scale molecular data in biodiversity studies. We combined a especific platform and library to the genome skimming and obtained an innovative approach, opening a new range of possibilities of its use in obtaining molecular data from organisms with different levels of preservation. The principal advantages from our approach are: i. it requires low amount of input DNA (0.2 ng/ul), which allows the use of organisms with ancient DNA; ii. it does not depends on specific primers and is not affected by gene rearrangement; and iii. it is time-saving and cost-effective, leading to high-quality complete sequence assemblies.