Affinities and melting temperatures (Tm) for the non-randomized albumin-binding domain (ABD), ADAPTERBB2-1 and variants of ADAPTERBB2-1.

Kinetic parameters are presented as mean values with standard deviation. KD was calculated from the rate constants (kd/ka) and the number of replicates for each variant is indicated within brackets. No binding to ERBB2 could be observed, even at elevated concentrations, for the negative control ABD or the four variants T7A, Y10A, H11A and Y38A. Melting temperatures (Tm) from CD measurements are also included.