SAIMOS - Biological and Flow Cytometry data collected from sampling stations in South Australia, in February 2021

Flow cytometry data was collected in February 2021, in waters off South Australia, from both the Upper Spencer Gulf (SAMUSG) and Gulf St Vincent (SAMGSV). The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.Since July 2019 two additional BGC stations have been added to the SAIMOS sampling array: the SA Upper Spencer Gulf Mooring (SAMUSG, 33.111S, 137.709E) and the SA Gulf St Vincent Mooring (SAMGSV, 35.023S, 138.46E).

本数据集的流式细胞术（Flow cytometry）数据采集于2021年2月，覆盖澳大利亚南部近海的斯宾塞湾上部（SAMUSG）与圣文森特湾（SAMGSV）海域。本研究的核心目标是为澳大利亚南部大陆架海域的生态系统建立基础背景资料，阐明上升流与高盐外流事件对微生物群落的影响，最终构建该区域的生物地球化学模型。本研究的采样工作依托澳大利亚南部综合海洋系统（SAIMOS）项目，在RV Ngerin科考船的多个航次中完成。各航次均对叶绿素荧光最大值层（DCM）的物理、化学与生物学特性开展了系统调查。本次研究采集了超微型浮游植物、细菌与病毒的流式细胞术数据。本研究共布设6个核心采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个站位为位于袋鼠岛西南侧的远海站位B1（36.188°S，136.178°E）。需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）与科考船平均航速（9节）可知，各站位采集的水体并非同一水团。研究期间还偶有采集袋鼠岛国家参考站（NRS，35.832°S，136.447°E）以及发生高盐外流事件的南澳斯宾塞湾口锚泊站位（SAM8SG，35.25°S，136.690°E）的样本。自2019年7月起，SAIMOS采样阵列新增2个生物地球化学（Biogeochemical, BGC）站位：南澳斯宾塞湾上部锚泊站位（SAMUSG，33.111°S，137.709°E）与南澳圣文森特湾锚泊站位（SAMGSV，35.023°S，138.46°E）。